Fig. 2.

T-bet expression in DCs is dependent on IFNγ. (A and B) Classical DCs (DX5^neg/CD11c^hi/MHC-II^hi) (A) and pDCs (DX5^neg/CD11c^Int/B220^hi) (B) were FACS-sorted (purity >95%) from the spleens of B6 and 129S/v WT, 129S/v Stat1^-/-, and B6 IFNγ^-/- mice. (C) B cells were positively selected with anti-CD19-coated magnetic beads from the spleens of WT and IFNγ^-/- mice and stimulated with CpG ODN (1 μg/ml) alone or in combination with exogenous IL-12 (10 ng/ml) and IFNγ (10 ng/ml). At the indicated times, mRNA was prepared from each sample for real-time PCR analysis. T-bet mRNA is expressed as copies per 10,000 mRNA copies of β-actin.