Table 2.
Virulence of H3 viruses in chickens.a
| Viruses | Virus shedding (log10EID50) (positive/total) | Death/total | Seroconversion (HI antibody titers) (positive/total) | |||||
|---|---|---|---|---|---|---|---|---|
| 2 dpi | 4 dpi | 6 dpi | ||||||
| Pharynx | Cloacae | Pharynx | Cloacae | Pharynx | Cloacae | |||
| DK/JX/E1137/2021(H3N8) | < | 2.75 (1/8) | < | 5.5 (1/8) | < | 1.25 (1/5) | 0/5 | 2 (1/5) |
| DK/GD/G1202/2022(H3N2) | 3.5 (1/8) | 3.5 (1/8) | 2.7 ± 0.90 (4/8) | 5.75 (1/8) | < | 1.25 (1/5) | 0/5 | 2–8 (4/5) |
| CK/NX/N1053/2022(H3N8) | 4.47 ± 0.86 | 1.25 (1/8) | 4.22 ± 1.72 | 1.46 ± 0.17 (7/8) | 1.75 ± 0.66 (3/5) | 2.83 ± 2.32 (3/5) | 0/5 | 8–32 (5/5) |
| GS/JX/E1427/2022(H3N8) | 4.78 ± 0.76 | 1.38 ± 0.18 (2/8) | 4.72 ± 0.34 | 1.38 ± 0.18 (2/8) | 1.5 ± 0.35 (2/5) | < | 0/5 | 16–32 (5/5) |
aGroups of eight chickens were inoculated with 106.0 EID50 per 0.1 ml of test virus. All chickens were monitored daily for clinical signs. Pharyngeal and cloacal swabs were taken from live chickens at 2, 4, 6, and 8 days postinfection (dpi). <, No virus was detected in this sample.