Table 1.
Detailed comparison of the whole-genome sequences of GNU-2353 and GNU-2377 and other lineage-representative PRRSV-2 strains.
| Identity with GNU-2353/GNU-2377 (%) | Lineages | L1F | L1A (NADC34-like) | L1C (NADC30-like) | L1J (formerly LKC) | L5 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GNU-2353 GNU-2377 | MN184C | NADC34 | ISU-10 | FJ0908 | JBNU-22-N02 | NADC30 | ISU-30 | SXSZ-2020 | JB15-N-P31-GB | CA-2 | KNU-12-KJ4 | VR-2332 | |
| Nucleotides (length) | |||||||||||||
| 5′-UTR (187/191) | 91.1 | 95.3/94.3 | 95.7/93.8 | 95.2/93.2 | 94.7/92.7 | 100/90.1 | 93.2/94.8 | 88.6/88.4 | 91.6/93.8 | 92.3/93.6 | 91.6/89.1 | 90.5/89.1 | 94.2/89.6 |
| ORF1a (7212/7119) | 78.9 | 80.9/82.1 | 87.6/78.6 | 87.0/78.4 | 86.4/78.3 | 99.0/79.2 | 82.5/89.5 | 81.9/82.1 | 81.3/87.4 | 80.9/91.0 | 78.4/80.2 | 77.8/79.7 | 78.3/76.6 |
| ORF1b (4377/4377) | 86.3 | 90.7/87.9 | 91.0/86.5 | 90.8/86.3 | 90.5/86.3 | 99.1/86.5 | 89.1/92.8 | 91.7/86.9 | 88.3/91.8 | 87.7/93.8 | 84.6/84.0 | 84.2/83.7 | 86.4/85.9 |
| ORF2−7 (3188/3188) | 86.1 | 87.5/86.1 | 95.6/87.8 | 95.5/87.8 | 94.2/86.6 | 99.2/86.3 | 89.0/92.6 | 88.0/91.1 | 89.0/92.3 | 87.7/94.1 | 85.7/84.4 | 86.1/84.7 | 86.9/85.5 |
| 3′-UTR (151/151) | 93.4 | 94.7/94.7 | 98.3/94.4 | 98.7/94.7 | 97.4/93.4 | 98.0/95.4 | 95.4/96.7 | 94.0/92.7 | 95.4/96.7 | 91.9/96.7 | 94.7/93.4 | 94.0/94.7 | 91.4/90.1 |
| Complete (15,109/15,020) | 82.9 | 85.4/84.9 | 90.5/83.2 | 90.1/83.0 | 89.4/82.7 | 99.0/83.1 | 86.0/91.2 | 86.2/85.6 | 85.2/89.9 | 84.5/92.6 | 82.1/82.5 | 81.7/82.2 | 82.7/81.4 |
| Amino acids (length) | |||||||||||||
| nsp1α (180/180) | 93.3 | 94.4/95.6 | 95.0/95.0 | 95.0/96.1 | 95.0/95.0 | 98.3/93.3 | 93.3/95.6 | 95.0/97.2 | 93.3/95.0 | 93.9/95.0 | 93.9/95.0 | 92.2/95.6 | 95.0/96.7 |
| nsp1β (203/203) | 72.9 | 82.8/79.8 | 89.7/73.4 | 89.7/73.4 | 86.7/75.9 | 99.0/72.9 | 78.8/82.3 | 88.2/74.4 | 78.8/82.3 | 74.4/85.7 | 76.4/76.8 | 78.8/74.4 | 77.8/82.8 |
| nsp2 (1096/1065) | 69.3 | 72.9/77.7 | 85.9/68.9 | 84.3/68.4 | 83.4/67.8 | 98.7/69.5 | 73.4/86.0 | 72.2/78.1 | 72.5/84.6 | 72.0/89.3 | 70.2/73.8 | 68.6/73.7 | 63.9/64.0 |
| nsp2TF (919/888) | 64.4 | 69.3/75.3 | 85.2/65.0 | 83.5/64.5 | 82.4/64.0 | 98.7/64.7 | 68.4/84.1 | 68.2/75.9 | 67.6/82.5 | 67.2/87.6 | 65.7/70.4 | 64.5/70.4 | 59.0/59.3 |
| nsp2N (750/719) | 57.7 | 64.3/72.2 | 83.5/58.9 | 81.3/58.4 | 80.3/58.0 | 98.4/58.1 | 62.8/81.9 | 63.2/72.6 | 61.7/80.0 | 61.2/85.8 | 60.7/66.8 | 59.3/66.8 | 53.5/53.9 |
| nsp3 (230/230) | 93.9 | 90.4/90.4 | 92.6/93.0 | 92.6/93.0 | 92.6/92.6 | 99.6/94.3 | 96.1/94.8 | 88.7/88.3 | 93.5/93.5 | 94.8/93.9 | 92.2/90.9 | 93.0/91.3 | 93.5/92.2 |
| nsp4 (204/204) | 91.7 | 90.2/91.2 | 90.7/91.7 | 90.7/91.7 | 90.7/91.7 | 99.5/92.2 | 93.1/96.1 | 90.2/93.1 | 92.2/95.1 | 92.6/98.0 | 88.2/89.7 | 90.2/91.7 | 93.1/93.6 |
| nsp5 (170/170) | 85.9 | 87.1/87.1 | 90.0/82.9 | 88.2/81.8 | 90.0/82.4 | 100/85.9 | 91.2/91.8 | 92.4/85.9 | 91.2/88.2 | 88.2/91.8 | 91.2/85.3 | 87.6/84.7 | 94.1/87.6 |
| nsp6 (16/16) | 100 | 100/100 | 93.8/93.8 | 93.8/93.8 | 87.5/87.5 | 100/100 | 100/100 | 100/100 | 100/100 | 100/100 | 100/100 | 100/100 | 100/100 |
| nsp7α (149/149) | 91.9 | 90.6/92.6 | 95.3/92.6 | 95.3/92.6 | 94.6/93.3 | 100/91.9 | 90.6/95.3 | 95.3/91.3 | 89.3/94.0 | 90.6/96.0 | 89.9/91.3 | 89.9/94.0 | 90.6/92.6 |
| nsp7β (110/110) | 74.5 | 84.5/80.0 | 90.9/77.3 | 91.8/77.3 | 90.9/78.2 | 98.2/74.5 | 77.3/88.2 | 86.4/73.6 | 78.2/88.2 | 76.4/90.0 | 73.6/70.9 | 70.9/70.9 | 81.8/77.3 |
| nsp8 (45/45) | 88.9 | 86.7/91.1 | 93.3/95.6 | 93.3/95.6 | 93.3/95.6 | 97.8/91.1 | 88.9/93.3 | 93.3/95.6 | 88.9/91.1 | 88.9/93.3 | 82.2/86.7 | 80.0/84.4 | 86.7/91.1 |
| nsp9 (685/685) | 94.3 | 96.5/95.6 | 97.1/95.9 | 96.9/95.8 | 96.1/95.0 | 99.3/94.6 | 95.9/97.4 | 97.1/95.3 | 95.9/97.2 | 95.2/97.5 | 94.3/94.2 | 94.7/94.2 | 95.3/95.6 |
| nsp10 (441/441) | 97.1 | 97.5/97.1 | 98.0/97.1 | 97.5/97.0 | 97.7/96.8 | 99.3/96.8 | 98.0/98.0 | 97.7/97.5 | 96.8/97.1 | 97.1/97.7 | 94.1/93.9 | 94.3/94.6 | 95.0/95.7 |
| nsp11 (223/223) | 91.9 | 92.8/92.4 | 94.2/94.6 | 93.3/93.7 | 93.7/94.2 | 98.7/91.9 | 94.2/95.5 | 94.2/92.8 | 94.2/93.3 | 93.3/96.9 | 92.8/91.9 | 92.8/92.4 | 94.6/93.7 |
| nsp12 (153/153) | 87.6 | 93.5/90.2 | 95.4/88.2 | 95.4/88.2 | 95.4/88.2 | 98.7/88.9 | 88.2/96.1 | 97.4/90.2 | 87.6/96.1 | 86.9/96.7 | 89.5/90.9 | 89.5/91.5 | 89.5/92.2 |
| GP2 (256/256) | 82.0 | 87.5/87.5 | 94.9/83.2 | 94.1/83.2 | 94.1/82.0 | 97.7/82.4 | 84.8/91.4 | 85.9/87.1 | 83.2/88.3 | 84.4/94.5 | 82.8/80.9 | 81.6/83.6 | 85.9/87.1 |
| E (73/73) | 89.0 | 90.4/93.2 | 97.3/89.0 | 97.3/89.0 | 97.3/89.0 | 100/89.0 | 90.4/93.2 | 90.4/93.2 | 91.8/89.0 | 87.7/95.9 | 89.0/86.3 | 89.0/89.0 | 87.7/87.7 |
| GP3 (254/254) | 77.6 | 79.5/81.1 | 93.3/81.5 | 92.9/81.1 | 89.0/78.0 | 97.6/79.5 | 82.3/89.4 | 82.7/88.2 | 82.3/89.4 | 78.0/90.2 | 79.5/78.7 | 82.7/79.1 | 81.9/79.9 |
| GP4 (178/178) | 92.7 | 87.1/86.5 | 97.2/93.8 | 97.2/94.4 | 92.1/89.9 | 97.8/93.3 | 95.5/93.8 | 93.3/92.7 | 95.5/93.8 | 96.1/94.9 | 88.2/87.6 | 87.6/86.5 | 86.5/87.1 |
| GP5a (46/46) | 91.3 | 93.5/91.3 | 97.8/93.5 | 97.8/93.5 | 97.8/93.5 | 100/91.3 | 93.5/93.5 | 91.3/95.7 | 93.5/93.5 | 89.1/95.7 | 95.7/91.3 | 84.8/84.8 | 87.0/84.8 |
| GP5 (200/200) | 88.5 | 90.5/87.0 | 97.0/88.0 | 96.0/88.0 | 95.0/87.5 | 99.5/88.0 | 91.5/89.5 | 90.5/88.0 | 91.5/89.5 | 90.0/92.0 | 90.5/86.5 | 90.5/85.0 | 84.5/81.5 |
| M (174/174) | 92.0 | 91.4/93.7 | 97.7/93.7 | 97.7/93.7 | 96.0/93.7 | 99.4/92.5 | 92.0/96.6 | 91.4/96.0 | 92.0/96.6 | 92.5/97.1 | 92.0/93.1 | 92.5/93.7 | 92.0/93.1 |
| N (123/123) | 92.7 | 90.2/91.9 | 96.7/93.5 | 96.7/93.5 | 96.7/91.9 | 100/92.7 | 97.6/95.1 | 94.3/93.5 | 97.6/95.1 | 95.9/96.7 | 90.2/91.9 | 91.9/91.1 | 91.9/93.5 |
Note: Bold values signify the nsps of VR-2332 with the highest amino acid identities compared to GNU-2353 and GNU-2377 in this study.