Table 2.
Primers or probes.
| Primers | Sequence (5′-3′) | Purpose |
|---|---|---|
| pCA-EcoR1-F | TCTCATCATTTTGGCAAAGAATTC | Point mutation |
| pCA-Bgl2-R | ATTTTTGGCAGAGGGAAAAAGATCT | |
| HLJVP2-D279N-F | CACCAGAGCTGTGGCCGCAAACAATGGGCTAACGGCCGGCAC | |
| HLJVP2-D279N-R | GTGCCGGCCGTTAGCCCATTGTTTGCGGCCACAGCTCTGGTG | |
| SHG19VP2-N279D-F | AGAGCTGTAGCTGCAGACAATGGGCTGACGG | |
| SHG19VP2-N279D-R | CCGTCAGCCCATTGTCTGCAGCTACAGCTCT | |
| PCA-HLJVP2-F | TCTCATCATTTTGGCAAAGAATTCGCCACCATGACAAACCTGCAAGATC | |
| PCA-HLJVP2-R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTAATCTGGAACATCGTATGGGTATGCTCCTGCAATCTTCAG | |
| IBDV-Probe | FAM-CGGCGTCCATTCCGGACGAC-BHQ-1 | RT-qPCR for IBDV |
| VP5-F | GAGCCTTCTGATGCCAACAAC | |
| VP5-R | CAAATTGTAGGTCGAGGTCTCTGA | |
| 28S-Probe | FAM-AGGACCGCTACGGACCTCCACCA-TAMRA | RT-qPCR for 28s rRNA |
| 28S-F | GGCGAAGCCAGAGGAAACT | |
| 28S-R | GACGACCGATTTGCACGTC | |
| IL-1β-F | CCGAGGAGCAGGGACTTT | RT-qPCR for IL-1β |
| IL-1β-R | AGGACTGTGAGCGGGTGTAG | |
| β-actin-F | TCCACCGCAAATGCTTCTAAAC | RT-qPCR for β-actin |
| β-actin-R | CTGCTGACACCTTCACCATTCC |