Fig. 4.

Isoproterenol and epothilone D protected neurons against tau overexpression-induced cell death. (A) Schematic diagram for evaluation of cell viability. Isoproterenol or epothilone D was added at the time of neuron plating. Neurons were treated with lentivirus (MOI 3) at 2 days after plating and cell viability was assessed 7 days after the lentivirus treatment. Neuronal viability of iPSC-derived neurons after exposure to tau aggregation inhibitor isoproterenol (B) and microtubule stabilizer epothilone D (C) were evaluated by CellTiter Glo assay (n = 3 ∼ 9, mean + SD). *: P ≤ 0.05, **: P ≤ 0.01, ***: P ≤ 0.001 by Dunnett’s test, 0 µM group vs. each group.