Fig. 2.

Overexpression of circHIPK2 promotes the proliferation and migration of LSCC cells. a: circHIPK2 overexpression plasmids were transfected into TU212 and TU686 cells, and the expression level of circHIPK2 was detected by qRT-PCR. b, c: Overexpression of circHIPK2 promoted colony-forming abilities of TU212 and TU686 cells. d, e: circHIPK2 was overexpressed, and cell proliferation was detected by CCK8 at a specified time point. f, g: Cell proliferation after circHIPK2 overexpression was detected by EdU staining. h, i: Overexpression of circHIPK2 improved the migration ability of TU212 and TU686 cells. j, k: Wound healing time of TU212 and TU686 cells after circHIPK2 overexpression was detected. l: In vivo experiment, a subcutaneous tumor formation model of nude mice was constructed with circHIPK2 overexpression and control cells. m, n: Comparison of tumor weight and volume between circHIPK2 overexpression and control cells. o: After circHIPK2 overexpression, the expression levels of migration marker proteins were detected by Western blotting. *P < 0.05, ^⁎⁎P < 0.01,^⁎⁎⁎P < 0.001.