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. 2025 Mar 11;103:skaf075. doi: 10.1093/jas/skaf075

Table 3.

Concentration of BAs in fecal samples from healthy adult dogs consuming 1 of 4 experimental diets with 0%, 15%, 30%, or 45% whole pulse ingredients (Ctl, Pulse15, Pulse30, Pulse45) after 20 wk of consumption, presented as least square means

Bile acid Treatment
(µmol/g) Ctl SEM Pulse15 SEM Pulse30 SEM Pulse45 SEM P-values
n 7 6 7 6 TRT1 L Q C
Primary BAs2
 Cholic acid 0.149 0.140 0.147 0.156 0.121 0.108 0.182 0.192 0.992 0.920 0.998 0.791
 Chenodeoxycholic acid 0.086 0.058 0.267 0.209 0.261 0.170 0.242 0.186 0.619 0.322 0.504 0.783
Total primary BAs 0.259 0.201 0.452 0.398 0.451 0.331 0.515 0.448 0.927 0.690 0.689 0.799
Secondary BAs
 Deoxycholic acid 3.304 1.066 8.213 2.962 6.403 2.140 4.062 1.465 0.261 0.054 0.951 0.774
 Lithocholic acid 0.882b 0.231 3.694a 1.018 2.826a 0.709 1.812ab 0.498 0.008 0.001 0.408 0.935
 Ursocholic acid 0.038 0.019 0.024 0.014 0.038 0.021 0.008 0.005 0.209 0.965 0.343 0.058
Total secondary BAs 4.701 1.382 12.966 4.199 10.074 3.013 6.431 2.082 0.131 0.022 0.922 0.814
Primary:Secondary 0.054 0.036 0.034 0.024 0.042 0.025 0.070 0.053 0.905 0.596 0.764 0.748
Total BAs3 5.384 1.689 14.372 5.106 11.831 3.901 8.451 3.002 0.209 0.045 0.711 0.986

1Treatment.

2Bile acids.

3Total BAs is summation of all BAs reported in this table and Table S1.

a,bDifferent letters within the same row indicate statistical significance (P < 0.05).

n = number of observations. Orthogonal contrasts; L = linear, Q = quadratic, C = cubic.