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. 2025 Mar 11;103:skaf075. doi: 10.1093/jas/skaf075

Table 4.

Fecal bile acid proportions from healthy adult dogs consuming 1 of 4 experimental diets with 0%, 15%, 30%, or 45% whole pulse ingredients (Ctl, Pulse15, Pulse30, Pulse45) after 20 wk of consumption, presented as least square means

Bile Acid Treatment
*%, of total BA1 Ctl SEM Pulse15 SEM Pulse30 SEM Pulse45 SEM P-values
n 7 6 7 6 TRT2 L Q C
Primary BA, % of total
 Cholic acid 2.473 1.774 1.046 0.809 1.025 0.676 1.881 1.504 0.779 0.388 0.851 0.784
 Chenodeoxycholic acid 1.602 0.737 1.930 0.940 2.228 0.931 2.531 1.296 0.911 0.849 0.563 0.802
 Total primary acids 4.550 2.570 3.267 1.923 3.795 1.938 5.412 3.397 0.934 0.634 0.795 0.792
Secondary BA, % of total
 Deoxycholic acid 64.588 4.672 62.142 5.302 57.004 4.097 50.288 4.138 0.204 0.979 0.066 0.267
 Lithocholic acid 15.065 3.213 24.599 5.751 24.571 4.830 21.219 5.072 0.421 0.175 0.482 0.962
 Ursocholic acid 0.741a 0.348 0.167ab 0.076 0.330ab 0.137 0.098b 0.045 0.037 0.129 0.154 0.019
Total secondary acids 92.093 11.531 96.169 7.786 90.633 10.633 83.481 6.013 0.674 0.542 0.354 0.671
Primary:Secondary 0.052 0.035 0.034 0.023 0.041 0.024 0.073 0.054 0.887 0.595 0.734 0.711

1Bile acids.

2Treatment.

a,bDifferent letters within the same row indicate statistical significance (P < 0.05).

*Percentage derived from the total BAs presented in Table 3 and Table S1.

n = number of observations. Orthogonal contrasts; L = linear, Q = quadratic, C = cubic.