Table 3. Transfected plasmid amounts for Nb37 bystander NanoBiT assays.

LgBiT-KRAS CAAX motif (SSSGGGKKKKKKSKTKCVIM)		Endofin FYVE domain-LgBiT(amino acid region Gln739-Lys806)
Plasmid	Concentration	Plasmid	Concentration
SNAP/FLAG-hGLP-1R WT or V229A	0.1 µg	SNAP/FLAG-hGLP-1R WT or V229A	0.1 µg
Nb37-SmBiT	0.05 µg	Nb37-SmBiT	0.25 µg
LgBiT-CAAX	0.05 µg	Endofin-LgBiT	0.25 µg
Gαs (human, short isoform)	0.25 µg	Gαs (human, short isoform)	0.25 µg
Gβ1 (human)	0.25 µg	Gβ1 (human)	0.25 µg
Gγ1 (human)	0.25 µg	Gγ1 (human)	0.25 µg
RIC8B (human, isoform 2)	0.05 µg	RIC8B (human, isoform 2)	0.05 µg
pcDNA 3.1	0.40 µg	pcDNA 3.1	-