Abstract
This paper describes the isolation and characterization of spontaneous inversion mutants of Salmonella typhimurium. The mutants are selected by demanding that an unexpressed hisD gene acquire a new promoter. Chromosome rearrangements that juxtapose the hisD gene and a foreign promoter are obtained by this selection. Although a number of inversions are found, the frequency was lower than expected. The breakpoints of these inversions are not distributed randomly either in the his operon or on the chromosome. The his breakpoint lies in the hisG-hisD intercistronic region, a sequence known to occur at several places on the bacterial chromosome. In most of the inversions, the "non-his" breakpoint lies across the chromosome, so that the inverted region includes the origin or terminus of DNA replication. The significance of these results is discussed.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnes W. M. Construction of an M13 histidine-transducing phage: a single-stranded cloning vehicle with one EcoRI site. Gene. 1979 Feb;5(2):127–139. doi: 10.1016/0378-1119(79)90098-2. [DOI] [PubMed] [Google Scholar]
- Casse F., Pascal M. C., Chippaux M. Comparison between the chromosomal maps of Escherichia coli and Salmonella typhimurium. Length of the inverted segment in the trp region. Mol Gen Genet. 1973 Aug 17;124(3):253–257. doi: 10.1007/BF00293096. [DOI] [PubMed] [Google Scholar]
- Ebel-Tsipis J., Botstein D., Fox M. S. Generalized transduction by phage P22 in Salmonella typhimurium. I. Molecular origin of transducing DNA. J Mol Biol. 1972 Nov 14;71(2):433–448. doi: 10.1016/0022-2836(72)90361-0. [DOI] [PubMed] [Google Scholar]
- Higgins C. F., Ames G. F., Barnes W. M., Clement J. M., Hofnung M. A novel intercistronic regulatory element of prokaryotic operons. Nature. 1982 Aug 19;298(5876):760–762. doi: 10.1038/298760a0. [DOI] [PubMed] [Google Scholar]
- Trowsdale J., Anagnostopoulos C. Differences in the genetic structure of Bacillus subitilis strains carrying the trpE26 mutation and strain 168. J Bacteriol. 1976 May;126(2):609–618. doi: 10.1128/jb.126.2.609-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]