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. 2025 Apr 24;15:14329. doi: 10.1038/s41598-025-94549-2

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — Oncogenic KRAS inactivation affects proteostasis. (A) Representative overlaid histograms for the fluorescence intensity of siControl and siKRAS HCT116 and SW837 cells. MG132 was used as positive control. (B) The mean fluorescence intensity values are used to compute the aggresome propensity factor (APF). Data shows the results of three biological replicates and are shown as mean ± SD. (C) Representative flow cytometry profiles for the fluorescence intensity of siControl and siKRAS HCT116 and SW837 cells. (D) The mean fluorescence intensity values of the positive population were used to compute the protein translation potential. Data shows the results of three independent experiments and are shown as mean ± SD. (E) Representative agarose gel of XBPsplicing inhibition after treatment with 4µ8C XBPsplicing and GAPDH PCR products were loaded in different gels. Uncropped versions of the images are provided in Supplementary Fig. 11. (F) Cell cycle analysis in HCT116 cells treated with 4µ8C. Bar graphs illustrate the percentage of cells G0/G1, S, G2/M. (G) Apoptosis analysis of HCT116 cells treated with 4µ8C. Bar graphs represent the percentage of cells that are in viable, early or late apoptosis, and necrotic states. Two-way ANOVA with multiple comparisons was used to compare different populations in siControl with siKRAS conditions (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 00001). All the bar graphs represent the mean ± SD of at least three independent experiments.