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. 2025 Apr 24;11:42. doi: 10.1186/s40798-025-00836-7

Table 4.

Intervention characteristics and outcomes of included animal studies

Author Type of exercise training Duration of exercise intervention Duration of each training session Frequency of exercise training Intensity of exercise training Outcomes (cognitive tests) Main cognitive results Effect size
Parsa et al. [98] Swimming 12 weeks 40 min 5 days/week ?

Cognitive memory (novel object recognition test and elevated plus maze)

Aversive memory and learning (passive avoidance learning test)

Swimming training alone insignificantly improved exploratory behaviour, locomotor activity, and passive avoidance memory, and non-spatial cognitive memory compared to sedentary diabetic rats /
Shekarchian et al. [99] Swimming 4 weeks

Week 1–2: 3 × 10 min

Week 3–4: 6 × 10 min

5 days/week ?

Working memory (Y maze)

Recognition memory (novel object recognition)

Spatial memory (MWM)

Exercise improved all memory aspects in exercising T2DM mice compared to non-exercising T2DM mice

Elevated plus maze test:

 Percentage of spontaneous alternation: η2 = 0.02

 Total arm entries: η2 = 0.01

Novel object recognition test:

 Familiar recognition ratio: η2 = 0.04

 Novel recognition ratio: η2 = 0.08

MWM:

 Escape latency: η2 = 0.10

 Platform quadrant time: η2 = 0.01

 Platform crossings: η2 = 0.01

Jesmin et al. [100] Treadmill running 4 months 30 min/day 5 days/week Light intensity: OLETF 7.0 m/min, LETO 10.0 m/min; Moderate intensity: OLETF 12.5 m/min, LETO 20 m/min Spatial learning memory and retention (MWM) Shortened swim length and escape latency in both light and moderate exercise groups improved memory

MWM:

 Escape latency: η2 = 0.11

 Swim length: η2 = 0.08

 Memory retention: η2 = 0.16

Lang et al. [101] Treadmill running 8 weeks 37 min/day 5 days/week Moderate intensity Spatial learning and memory (MWM) Compared with T group, the escape latency of E group was significantly reduced on day 4–5, treadmill exercise significantly increased the number of platform crossings in E group

MWM:

 Escape latency: d = − 1.39

 Platform quadrant time: d = 1.01

 Platform crossings: d = 1.32

Shima et al. [102] Treadmill running 4 weeks 30 min/day 5 days/week Moderate intensity (OLETF rats, 12.5 m/min; LETO rats, 20 m/min) Memory (MWM)

Escape latency and swim path length of exercising OLETF rats were shortened, without alteration of the speed of swimming, at trials on days 2, 3 and 4

The time spent by OLETF rats in the platform area significantly improved after 4 weeks of exercise

MWM:

 Escape latency: η2 = 0.11

 Swim length: η2 = 0.15

 Platform quadrant time: η2 = 0.16

Shima et al. [103] Running on a forced exercise wheel bed 4 weeks 30 min/day 5 days/week Light intensity Memory (MWM) No effect of exercise on the escape latency, swim distance or speed in ob/ob mice. The times of crossing the target platform during the probe test in C57BL/6 mice were significantly greater than that in sedentary ob/ob mice, but not than that in exercised ob/ob mice

MWM:

 Swim length: η2 = 0.02

 Swim duration: η2 = 0.05

E group diabetes + exercise group, LETO Long Evans Tokushima Otsuka, MWM Morris Water Maze, ob/ob obese-hyperglycemic, OLETF Otsuka Long-Evans Tokushima Fatty, T group diabetes group, T2DM type 2 diabetes