Fig. 2.
(a) Representative SDS-PAGE for BSA recovery from harvest and permeate fractions. (b) Quantitation of SDS-PAGE results for BSA transmission to permeate during TFF. Data split over two plots to improve clarity due to similarity of results. (c) Endotoxin concentration in starting material (SM), separate permeate fraction, and final retentate. Legend in (d) applies to (c). (d) Endotoxin in permeate as a percentage of total endotoxin in SM (representing 100%). (e) Representative cuvettes used for measuring yellow colour absorbance at 580 nm. (f) Absorbance of harvest supernatant solution scanned throughout visible wavelengths of light. Buffer used as blank for every wavelength. Violet and yellow lines indicate the regions for the wavelengths of these colours; 420 nm and 580 nm, respectively. (g) Linearity of absorbance at 420 nm (A420nm) using harvest supernatant dilution series, from two independent fermentation runs is shown. Representative permeate samples are shown and were measured undiluted. (h) Quantitation of A420nm for each permeate fraction, expressed as percent reduction of harvest supernatant. Data split over two plots to improve clarity due to similar results. Abbreviations: BSA = bovine serum albumin; Perm = permeate.