Table 2.

RNA thrapy targeting circRNA-RBP system.

RNA thrapy	RNA tool	Target gene	Methods	Applications	Refs
RNAi	siRNA , shRNA	circRNA	target circRNA-specific BSJ structure	Knockdown circRNAs in vivo.	91, 97
		RBP	control post-transcriptional gene expression through homology-dependent degradation of target mRNA by siRNA	Targeting nanoparticle delivery of HUR-RNAi to inhibit cancer development	98,99
RNA editing	ADARs;	RBP	gRNA for site-specific editing of mRNA	Treating A1-antitrypsin deficiency caused by SERPINA1 missense mutations.	90,100 103,104
		circRNA	circular ADAR-recruiting guide RNA	To improve RNA editing efficiency and extend the editing time.	90
CRISPR-Cas system	CRISPR/ Cas9	circRNA	disrupting intron pairing on both sides of the circular exon	Knocking out circHIPK3 in vitro.	107,108
	CRISPR/ Cas13	circRNAs	target circRNA-specific BSJ structure	Knock down the circRNA	89
	CRISPR/ Cas9	RBP	identify RBP molecules by high-throughput CRISPR screening	HNRNPL, METTL3, RBP network in AML	92,110,111