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Observed versus expected for a dilution series measured in the COBAS Amplicor HCV Monitor version 2.0 international unit assay. The means of triplicate values are calculated and plotted as log10 international units of HCV RNA per milliliter. The equation for the linear regression analysis is as follows: observed = 0.886(expected) + 0.437; R2 = 0.983; n = 30. The dashed line represents unity.
