TABLE 1.
Comparison of physical status of HPV-16 DNA in invasive cervical cancers analyzed by four different molecular methods
| Sample no. | E2/E6 ratioa | Result of:
|
|
|---|---|---|---|
| Real-time PCR | Southern hybridization and multiplex PCR | ||
| 1 | 0.53 | Mixedb | Episomal |
| 2 | 1.00 | Episomal | Episomal |
| 3 | 0.41 | Mixedb | Mixed |
| 4 | 1.11 | Episomal | Episomal |
| 5 | 0.91 | Episomal | Episomal |
| 6 | 0.94 | Episomal | Episomal |
| 7 | 0.55 | Mixedb | Episomal |
| 8 | 0.77 | Episomal | Episomal |
| 9 | 0.61 | Episomal | Episomal |
| 10 | 0.51 | Mixedb | Mixed |
| 11 | 0.87 | Episomal | Episomal |
| 12 | 1.13 | Episomal | Episomal |
a
Obtained by comparison of copy numbers of genes.
b
Concomitant form of HPV-16 DNA was confirmed by two-dimensional gel electrophoresis.