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. 2025 Mar 25;83:103615. doi: 10.1016/j.redox.2025.103615

Fig. 6.

Fig. 6

GPX4 deficiency triggered the fibrotic phenotype of endometrial epithelial organoids. (A) Scheme for stimulation of endometrial organoids. Organoids were passaged and plated on day 0 (d0) in expansion medium (ExM). On d4, ExM was changed to different medium for 8 d. ExM: EEOs established in ExM continuously cultured. Prol: EEOs were primed with E2 to mimic proliferative endometrium. Sec: EEOs were primed with P4 + cAMP to mimic secretory endometrium. Mid-sec: EEOs were primed with differentiation medium (P4 + cAMP + hCG + PRL + hPL) to mimic endometrium during pregnancy. Created with BioRender.com (https://biorender.com/). (B) Typical bright field images of organoids with different generations (upper) and cultivation days (lower). (C) The protein expression of fibronectin, GPX4, Smad2/3 and phosphorylated Smad2/3 in EEOs from non-PCOS patient (donor 1) treated with or without FIN56. (D) IF staining of EEOs for fibronectin between non-PCOS patient (upper) and non-PCOS patient treated with FIN56 (lower). Scale bar, 20 μm. (E) IF staining of organoid for phosphorylated Smad2/3 between non-PCOS patient (upper) and non-PCOS patient treated with FIN56 (lower). Scale bar, 20 μm.