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Detection by RT-PCR of SEG and SEI mRNAs in S. aureus isolates harboring seg and sei genes. Total RNAs purified at the mid-log phase from cultures of S. aureus isolates harboring seg and sei were subjected to RT-PCR analysis. Lanes: F1, Fukuoka 1; S1, Saga 1; A1, Aomori 1; M, HaeIII-digested φX174 as a size marker; PC, genomic DNA of Fukuoka 1 as a positive control; NC, Milli-Q water as a negative control; +, with RT; −, without RT.
