TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Genotype | Origin |
|---|---|---|
| S. aureus | ||
| Type strains | ||
| FRI722 | sea sed | M. S. Bergdoll |
| S6 | sea seb | M. S. Bergdoll |
| FRI361 | sec2 sed seg sei | M. S. Bergdoll |
| 1151-7NG | sed | M. S. Bergdoll |
| FRI326 | see | M. S. Bergdoll |
| FRI569 | seh | Y.-C. Su |
| Isolates | ||
| Nagasaki 1 | sea seb seh | Food poisoning outbreak |
| Fukuoka 2 | seg sei | Food poisoning outbreak |
| E. coli strains | ||
| DH5α | Toyobo | |
| KGX1 | Aprseg, carrying pKGX1 | This work |
| KHX1 | Aprseh, carrying pKHX1 | This work |
| KIX1 | Aprsei, carrying pKIX1 | This work |
| Plasmids | ||
| pGEM3Zf (+) | Apr | Promega |
| pGEX-6P-1 | Apr | Pharmacia |
| pKOG4 | Apr, pGEM3Zf(+) with seg | This work |
| pKOH1 | Apr, pGEM3Zf(+) with seh | This work |
| pKOI6 | Apr, pGEM3Zf(+) with sei | This work |
| pKGX1 | Apr, pGEX-6P-1 with seg | This work |
| pKHX1 | Apr, pGEX-6P-1 with seh | This work |
| pKIX1 | Apr, pGEX-6P-1 with sei | This work |