Representative profiles of probe reactivities with European samples. The genotype determined by DEIA is given at the top, and the reference subtype is given at the bottom. (A and B) The first screening discriminated subtype A-like, CRF01_AE, and subtype BD, C, F, and G samples. (C) Specific identification of subtypes B and D from the samples reactive with subtype BD-specific probes was performed in a second step with individual B- and D-specific probes. abs., absorbance.