TABLE 1.
Probe | Subtype(s) identified | Oligonucleotide sequencea | Tm (°C) |
---|---|---|---|
SAG1 | CRF02_AG | 5′-ATA TTT TGG ATA ATG ATA ATT TGT AAT GC-3′ | 53.7 |
SA2 | CRF02_AG, A, CRF01_AE | 5′-AAA GAY GCA GAG ACC ACC C-3′ | 55.2 |
SAE1 | CRF01_AE | 5′-AGA CAC AGA TGA ATT GGC CAA-3′ | 53.9 |
SB2 | B | 5′-GAM GGR GAY CAG GAA GAA TT-3′ | 51.2 |
SBD3 | B, BD, D | 5′-ATG CTC CTT GGG ATR TTR ATG-3′ | 52 |
SD1.1 | D | 5′-AGA GTG AGG GRG ATA GAG AGG AAT T-3′ | 59.5 |
SC1 | C | 5′-ATC TTA GGC TTT TGG ATG ITA ATG-3′ | 54 |
SF6 | F | 5′-GGG AAA TGG GGC CTT TTA TT-3′ | 53.2 |
SG1 | G | 5′-CAT ATA GTA CTG AAA GCC ATA ATG T-3′ | 54.6 |
Degenerate bases (in boldface)are defined as follows: Y, C + T; R, = A + G; M, A + C; I, inosine.