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. 2002 Mar;40(3):1010–1022. doi: 10.1128/JCM.40.3.1010-1022.2002

TABLE 1.

Characteristics of subtype-specific probes

Probe Subtype(s) identified Oligonucleotide sequencea Tm (°C)
SAG1 CRF02_AG 5′-ATA TTT TGG ATA ATG ATA ATT TGT AAT GC-3′ 53.7
SA2 CRF02_AG, A, CRF01_AE 5′-AAA GAY GCA GAG ACC ACC C-3′ 55.2
SAE1 CRF01_AE 5′-AGA CAC AGA TGA ATT GGC CAA-3′ 53.9
SB2 B 5′-GAM GGR GAY CAG GAA GAA TT-3′ 51.2
SBD3 B, BD, D 5′-ATG CTC CTT GGG ATR TTR ATG-3′ 52
SD1.1 D 5′-AGA GTG AGG GRG ATA GAG AGG AAT T-3′ 59.5
SC1 C 5′-ATC TTA GGC TTT TGG ATG ITA ATG-3′ 54
SF6 F 5′-GGG AAA TGG GGC CTT TTA TT-3′ 53.2
SG1 G 5′-CAT ATA GTA CTG AAA GCC ATA ATG T-3′ 54.6
a

Degenerate bases (in boldface)are defined as follows: Y, C + T; R, = A + G; M, A + C; I, inosine.