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Expression of MtuPps1 and MtuRecA inteins in E. coli. Tagged inteins were detected by Western blotting of the SDS-PAGE (10% polyacrylamide) gels used to separate proteins from soluble (S) and insoluble (I) fractions of MtuPps1 and MtuRecA extracts and immunologic detection by using anti-V5 antibody.
