TABLE 2.
Universal and Madurella-specific primers used for PCR and sequencinga
| Primer | Position (gene/nucleotides) | Reference | Primer sequence (5′ → 3′)b |
|---|---|---|---|
| ITS4 | LSU/41-60 | 23 | ←TCCTCCGCTTATTGATATGC |
| ITS5 | SSU/1745-1767 | 23 | GGAAGTAAAAGTCGTAACAAGG→ |
| V9D | SSU/1609-1627 | 6 | TTAAGTCCCTGCCCTTTGTA→ |
| LS266 | LSU/287-266 | 19 | ←GCATTCCCAAACAACTCGACTC |
| NS1 | SSU/20-38 | 23 | GTAGTCATATGCTTGTCT→ |
| NS24 | SSU/1769-1750 | 10 | ←AAACCTTGTTACGACTTTTA |
| Oli5 | SSU/83-102 | 14 | GAAACTGCGAATGGCTCATT→ |
| Oli9 | SSU/573-591 | 14 | CGCGGTAATTCCAGCTCCA→ |
| Oli10 | SSU/951-935 | 14 | ←TGGYRAATGCTTTCGC |
| Oli11 | SSU/962-980 | 14 | TTRATCAAGAACGAAAGT→ |
| Oli13 | SSU/1418-1438 | 14 | ←ATAACAGGTCTGTGATGCCC |
| Oli14 | SSU/1418-1438 | 14 | ATAACAGGTCTGTGATGCCC→ |
| 26.1Ac | ITS2/111-130 | 2 | ←AATGAGTTGGGCTTTAACGG |
| 28.3Ac | ITS1/474-494 | 2 | TCCCGGTAGTGTAGTGTCCCT→ |
a
Positions correspond to the Saccharomyces cerevisiae consensus sequence.
b
R = purine A or G; Y = pyrimidine C or T.
c
Primer sequences and positions are based on the M. mycetomatis ITS sequence (GenBank accession no. AF162133).