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The effect of LPS on BET protein expression in BV2 microglial cells. BV2 cells were treated with LPS (100 ng/mL) for 6 h. (a) mRNA levels of Brd2, Brd3, and Brd4 were analyzed using qPCR. (b) Protein levels of BET proteins were measured using ELISA assays. Data are presented as mean ± SEM; n = 4 (a) and 3–4 (b); * p < 0.05 and ** p < 0.01, compared to the control (Student’s t-test).
