FIG. 1. .

Optimization of oligoprobe concentration for RLB. (Upper panel) Different concentrations of oligoprobes were spotted onto the membrane and subsequently hybridized with GP5+/6+ PCR products derived from clinical specimens containing HPV type 16, HPV types 16 and 33, HPV types 11 and 18, HPV type 16, no HPV (−), HPV type 33, and HPV type 16 from (top to bottom). Optimal signals were derived when 100 to 200 pmol of oligoprobe/μl was used. (Lower panel) Dilutions of cloned HPV type 16 (pHPV16) DNA were subjected to GP5+/6+ PCR and RLB, showing that 100 ag of HPV type 16 DNA can easily be detected.