TABLE 2.
HPV type-specific oligoprobes used for EIA and RLB typing of GP5+/6+ PCR products
| HPV type | Sequence of oligoprobe for:
|
|
|---|---|---|
| EIA | RLBa | |
| 6 | ATCCGTAACTACATCTTCCACATACACCAA | TCCGTAACTACATCTTCCA |
| 11 | ATCTGTGTCTAAATCTGCTACATACACTAA | TCTGTGTCTAAATCTGCTAC |
| 16 | GTCATTATGTGCTGCCATATCTACTTCAGA | CATTATGTGCTGCCATATC |
| 18 | TGCTTCTACACAGTCTCCTGTACCTGGGCA | TGCTTCTACACAGTCTCCT |
| 26 | AGTACATTATCTGCAGCATCTGCATCCACT | GTACATTATCTGCAGCATC |
| 31 | TGTTTGTGCTGCAATTGCAAACAGTGATAC | GCAATTGCAAACAGTGATAC |
| 33 | TTTATGCACACAAGTAACTAGTGACAGTAC | TGCACACAAGTAACTAGTGA |
| 34 | TACACAATCCACAAGTACAAATGCACCATA | TTTTCAGTTTGTGTAGGTACA |
| 35 | GTCTGTGTGTTCTGCTGTGTCTTCTAGTGA | CTGCTGTGTCTTCTAGTGA |
| 39 | TCTACCTCTATAGAGTCTTCCATACCTTCT | ATAGAGTCTTCCATACCTTC |
| 40 | GCTGCCACACAGTCCCCCACACCAACCCCA | AGTCCCCCACACCAACC |
| 42 | CTGCAACATCTGGTGATACATATACAGCTG | TGGTGATACATATACAGCTG |
| 43 | TCTACTGACCCTACTGTGCCCAGTACATAT | TCTACTGACCCTACTGTG |
| 44 | GCCACTACACAGTCCCCTCCGTCTACATAT | TACTAGTGAACAATATAAGCA |
| 45b | ACACAAAATCCTGTGCCAAGTACAT | TAATTTAACATTATGTGCCTC |
| 51 | AGCACTGCCACTGCTGCGGTTTCCCCAACA | TGCTGCGGTTTCCCCAA |
| 52 | TGCTGAGGTTAAAAAGGAAAGCACATATAA | GAATACCTTCGTCATGGC |
| 53 | GTCTATGTCTACATATAATTCAAAGCAAAT | TGTCTACATATAATTCAAAGC |
| 54 | TACAGCATCCACGCAGGATAGCTTTAATAA | CACGCAGGATAGCTTTAAT |
| 55 | CTGCTACAACTCAGTCTCCATCTACAACAT | TCAGTCTCCATCTACAACAT |
| 56 | GTACTGCTACAGAACAGTTAAGTAAATATG | CAGAACAGTTAAGTAAATATG |
| 57 | CCACTGTAACCACAGAAACTAATTATAAAG | CCACAGAAACTAATTATAAAG |
| 58 | ATTATGCACTGAAGTAACTAAGGAAGGTAC | TATGCACTGAAGTAACTAAG |
| 59 | TCTACTACTGCTTCTATTCCTAATGTATAC | TCTACTACTGCTTCTATTCC |
| 61 | TACTGCTACATCCCCCCCTGTATCTGAATA | CCCCCCCTGTATCTGAAT |
| 66 | TATTAATGCAGCTAAAAGCACATTAACTAA | AGCTAAAAGCACATTAACTAA |
| 68 | TCTACTACTACTGAATCAGCTGTACCAAT | CTGAATCAGCTGTACCAAT |
| 70 | CTGCCTGCACCGAAACGGCCATACCTGCTG | GAAACGGCCATACCTGCT |
| 72 | TGCCACAGCGTCCTCTGTATCAGAATATAC | AGCGTCCTCTGTATCAGAA |
| 73 | GTGTAGGTACACAGGCTAGTAGCTCTACTA | ACAGGCTAGTAGCTCTACT |
| 82/MM4 | CGACTCGTGTTACTCAATCTGTTGCACAAA | ACTGCTGTTACTCAATCTG |
| 83 (MM7) | CAGCTGCTGCTACACAGGCTAATGAATACA | TACACAGGCTAATGAATACA |
| 84 (MM8) | GTGCTGCTACCAACACCGAATCAGAATATA | TGCTACCAACACCGAATCA |
| 82/IS39 | GCACTGCTGCTACTCCATCAGTTGCACAGA | TGCTACTCCATCAGTTGC |
| CP6108 | GTGCTGCTTCCCAGTCTGCCACAGAATACA | CTTCCCAGTCTGCCACA |
| 71 (CP8061) | GTGCTACCAAAACTGTTGAGTCTACATATA | TGCTACCAAAACTGTTGAG |
| 81 (CP8304) | GCACAGCTACATCTGCTGCTGCAGAATACA | GCTACATCTGCTGCTGC |
a
Except for HPV types 34, 44, 45, and 52, oligoprobes for RLB were selected from the oligoprobe sequence used in EIA and underlined in the EIA oligoprobe sequence.
b
Modified from a previously published sequence (8).