TABLE 3.
Oligonucleotide sequences used in the HPV E7 type-specific PCR
| Primer | Sequence (5′-3′) | Probe | Sequence (5′-3′) |
|---|---|---|---|
| HPV16E7.667Bio | GATGAAATAGATGGTCCAGC | 16E7DIGPRO | CGGACAGAGCCCATTACAATATTGTAACCT |
| HPV16E7.774Bio | GCTTTGTACGCACAACCGAAGC | ||
| HPV18E7.696 | AAGAAAACGATGAAATAGATGGA | 18E7DIGPRO | CCCGACGAGCCGAACCACAACGTCACACAA |
| HPV18E7.799Bio | GGCTTCACACTTACAACACA | ||
| HPV31E7.811 | GGGCTCATTTGGAATCGTGTG | 31E7DIGPRO | TACCTGCTGGATCAGCCATTGTAGTTACAG |
| HPV31E7.890Bio | AACCATTGCATCCCGTCCCC | ||
| HPV33E7.671Bio | TGAGGATGAAGGCTTGGACC | 33E7DIGPRO | TGTGACAACAGGTTACAATGTAGTAATCAG |
| HPV33E7.761 | TGACACATAAACGAACTGTG | ||
| HPV35E7.674Bio | CTATTGACGGTCCAGCT | 35E7DIGPRO | CAACAGGACGTTACAATATTATAATTGGAG |
| HPV35E7.752 | TACACACAGACGTAGTGTCG | ||
| HPV39E7.601Bio | CCAAAGCCCACCTTGCAGGA | 39E7DIGPRO | TCCTAATTGCTCGTGACATACAAGGTCAAC |
| HPV39E7.703 | ATGGTCGGGTTCATCTATTTC | ||
| HPV45E7.741Bio | CCCACGAGCCGAACCACAG | 45E7DIGPRO | AGCTCAATTCTGCCGTCACACTTACAACAT |
| HPV45E7.822 | TCTAAGGTCCTCTGCCGAGC | ||
| HPV51E7.718 | TACGTGTTACAGAATTGAAG | 51E7DIGPRO | TCAAGTGTAGTACAACTGGCAGTGGAAAGC |
| HPV51E7.841Bio | AACCAGGCTTAGTTCGCCCATT | ||
| HPV52E7.691Bio | GCAGAACAAGCCACAAGCAA | 52E7DIGPRO | ATAGCCGTAGTGTGCTATCACAACTGTGAC |
| HPV52E7.776 | TAGAGTACGAAGGTCCGTCG | ||
| HPV56E7.784 | GGTGCAGTTGGACATTCAGAG | 56E7DIGPRO | CAAAGAGGACCTGCGTGTTGTACAACAGCT |
| HPV56E7.886Bio | GTTACTTGATGCGCAGAGTG | ||
| HPV58E7.98 | CGAGGATGAAATAGGCTTGG | 58E7DIGPRO | ACAGCTAATTACTACATTGTAACTTGTTGT |
| HPV58E7.761Bio | ACACAAACGAACCGTGGTGC | ||
| HPV59E7.646 | CTCCGAGAATGAAAAAGATGAA | 59E7DIGPRO | ATCATCCTTTGCTACTAGCTAGACGAGCTG |
| HPV59E7.749Bio | GCTGAAGTTGATTATTACA | ||
| HPV66E7.641 | AATGCAATGAGCAATTGGACAG | 66E7DIGPRO | AGGATGAAATAGACCATTTGCTGGAGCGGC |
| HPV66E7.742Bio | CTTATGTTGTTCAGCTTGTC | ||
| HPV68E7.4604 | AACAACAGCGTCACACAATTCA | 68E7DIGPRO | AGTGTAACAACCTACTGCAACTAGTAGTAG |
| HPV68E7.4704Bio | AGTTGTACGTTCCGCAGGTT |