Table 3.

Evaluation of the precision of the developed HPLC-PDA method for the detection and quantification of 20E, TU, and PA.

Concentration (μg/mL)	Intra-Day Precision			Inter-Day Precision
	Mean (μg/mL ± SD)	SEM	CV%	Mean (μg/mL ± SD)	SEM	CV%
Turkesterone
40	40.22 ± 0.51	0.01	1.17	40.30 ± 0.13	0.01	0.32
25	25.08 ± 0.30	0.01	1.18	25.08 ± 0.17	0.01	0.66
10	9.94 ± 0.02	0.01	0.25	10.01 ± 0.13	0.01	1.34
20-Hydroxyecdysone
40	40.22 ± 0.09	0.04	0.23	40.06 ± 0.11	0.04	0.27
25	25.06 ± 0.15	0.07	0.62	25.17 ± 0.19	0.08	0.77
10	9.98 ± 0.05	0.02	0.54	10.00 ± 0.07	0.03	0.72
Ponasterone A
40	40.53 ± 0.29	0.12	0.73	40.61 ± 0.43	0.17	1.06
25	24.99 ± 0.09	0.04	0.37	25.08 ± 0.17	0.08	0.66
10	9.96 ± 0.04	0.01	0.39	9.94 ± 0.04	0.01	0.36