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. 2025 Mar 31;17(4):501. doi: 10.3390/v17040501

Figure 1.

Figure 1

Analysis of UL111A cmvIL-10 and LAcmvIL-10 transcripts in permissive infected MRC-5 cells and during the establishment of latency in CD14+ monocytes. MRC-5 (A) and CD14+ monocytes (B) were infected with HCMV TB40E strain at MOI 1 and 5, respectively; cmvIL10 and LAcmvIL-10 RNA levels were quantified by RT-qPCR and are expressed as fold change relative to housekeeping gene GAPDH (on the Y-axis) at different time points: 24, 48, 72, and 96 hpi (on the X-axis). For viral reactivation in CD14+ monocytes, phorbolmyristate acetate (PMA) was added to the media at 96 hpi and left until 120 hpi. Each bar represents the means with standard deviations of fold changes in expression of the experimental results of three biological and two technical repeats for each time point (expression in CD14+ monocytes was investigated from 2 different donors, and bars represent the mean of both sets of data).