Abstract
A mutation abolishing photorepair has been localized to map position 56.8 centimorgans on the second chromosome of Drosophila melanogaster . Strains homozygous for the phr allele are totally devoid of photorepair and partially deficient in excision repair. Both defects map to the chromosomal region between pr and c. Since a homozygous phr stock exhibits reduced photoreactivation, the corresponding wild-type allele plays a significant role in UV resistance.
Full Text
The Full Text of this article is available as a PDF (715.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boyd J. B., Golino M. D., Shaw K. E., Osgood C. J., Green M. M. Third-chromosome mutagen-sensitive mutants of Drosophila melanogaster. Genetics. 1981 Mar-Apr;97(3-4):607–623. doi: 10.1093/genetics/97.3-4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyd J. B., Harris P. V. Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster. Chromosoma. 1981;82(2):249–257. doi: 10.1007/BF00286109. [DOI] [PubMed] [Google Scholar]
- Boyd J. B., Harris P. V., Osgood C. J., Smith K. E. Biochemical characterization of repair-deficient mutants of Drosophila. Basic Life Sci. 1980;15:209–221. doi: 10.1007/978-1-4684-3842-0_14. [DOI] [PubMed] [Google Scholar]
- Boyd J. B., Shaw K. E. Postreplication repair defects in mutants of Drosophila melanogaster. Mol Gen Genet. 1982;186(2):289–294. doi: 10.1007/BF00331864. [DOI] [PubMed] [Google Scholar]
- Brown T. C., Harris P. V., Boyd J. B. Effects of radiation on the survival of excision-defective cells from Drosophila melanogaster. Somatic Cell Genet. 1981 Nov;7(6):631–644. doi: 10.1007/BF01538753. [DOI] [PubMed] [Google Scholar]
- Chan L. N., Gehring W. Determination of blastoderm cells in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2217–2221. doi: 10.1073/pnas.68.9.2217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferro W. Studies on mutagen-sensitive strains of Drosophila melanogaster. V. Biochemical characterization of a strain (ebony) that is UV- and X-ray sensitive and deficient in photorepair. Mutat Res. 1985 May;149(3):399–408. doi: 10.1016/0027-5107(85)90156-3. [DOI] [PubMed] [Google Scholar]
- Harris P. V., Boyd J. B. Pyrimidine dimers in Drosophila chromatin become increasingly accessible after irradiation. Mutat Res. 1987 Jan;183(1):53–60. doi: 10.1016/0167-8817(87)90045-9. [DOI] [PubMed] [Google Scholar]
- Maurer R., Osmond B. C., Botstein D. Genetic analysis of DNA replication in bacteria: dnaB mutations that suppress dnaC mutations and dnaQ mutations that suppress dnaE mutations in Salmonella typhimurium. Genetics. 1984 Sep;108(1):25–38. doi: 10.1093/genetics/108.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Resnick M. A. A photoreactivationless mutant of Saccharomyces cerevisiae. Photochem Photobiol. 1969 Apr;9(4):307–312. doi: 10.1111/j.1751-1097.1969.tb07294.x. [DOI] [PubMed] [Google Scholar]
- Ryo H., Kondo S. Photoreactivation rescue and hypermutability of ultraviolet-irradiated excisionless Drosophila melanogaster larvae. Proc Natl Acad Sci U S A. 1986 May;83(10):3366–3370. doi: 10.1073/pnas.83.10.3366. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sancar A., Franklin K. A., Sancar G. B. Escherichia coli DNA photolyase stimulates uvrABC excision nuclease in vitro. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7397–7401. doi: 10.1073/pnas.81.23.7397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow R. B., Setlow J. K. Effects of radiation on polynucleotides. Annu Rev Biophys Bioeng. 1972;1:293–346. doi: 10.1146/annurev.bb.01.060172.001453. [DOI] [PubMed] [Google Scholar]
- Sutherland B. M., Rice M., Wagner E. K. Xeroderma pigmentosum cells contain low levels of photoreactivating enzyme. Proc Natl Acad Sci U S A. 1975 Jan;72(1):103–107. doi: 10.1073/pnas.72.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto K., Satake M., Shinagawa H. A multicopy phr-plasmid increases the ultraviolet resistance of a recA strain of Escherichia coli. Mutat Res. 1984 Jan;131(1):11–18. doi: 10.1016/0167-8817(84)90042-7. [DOI] [PubMed] [Google Scholar]
- Yegorova L. A., Levin V. L., Kozlova M. A. Heritability of UV-sensitivity and photo-reactivation ability in Drosophila embryos. Mutat Res. 1978 Feb;49(2):213–218. doi: 10.1016/0027-5107(78)90159-8. [DOI] [PubMed] [Google Scholar]