Skip to main content
Genetics logoLink to Genetics
. 1987 Jun;116(2):233–239. doi: 10.1093/genetics/116.2.233

Isolation and Characterization of a Photorepair-Deficient Mutant in Drosophila melanogaster

James B Boyd 1, Paul V Harris 1
PMCID: PMC1203134  PMID: 3111935

Abstract

A mutation abolishing photorepair has been localized to map position 56.8 centimorgans on the second chromosome of Drosophila melanogaster . Strains homozygous for the phr allele are totally devoid of photorepair and partially deficient in excision repair. Both defects map to the chromosomal region between pr and c. Since a homozygous phr stock exhibits reduced photoreactivation, the corresponding wild-type allele plays a significant role in UV resistance.

Full Text

The Full Text of this article is available as a PDF (715.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd J. B., Golino M. D., Shaw K. E., Osgood C. J., Green M. M. Third-chromosome mutagen-sensitive mutants of Drosophila melanogaster. Genetics. 1981 Mar-Apr;97(3-4):607–623. doi: 10.1093/genetics/97.3-4.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyd J. B., Harris P. V. Mutants partially defective in excision repair at five autosomal loci in Drosophila melanogaster. Chromosoma. 1981;82(2):249–257. doi: 10.1007/BF00286109. [DOI] [PubMed] [Google Scholar]
  3. Boyd J. B., Harris P. V., Osgood C. J., Smith K. E. Biochemical characterization of repair-deficient mutants of Drosophila. Basic Life Sci. 1980;15:209–221. doi: 10.1007/978-1-4684-3842-0_14. [DOI] [PubMed] [Google Scholar]
  4. Boyd J. B., Shaw K. E. Postreplication repair defects in mutants of Drosophila melanogaster. Mol Gen Genet. 1982;186(2):289–294. doi: 10.1007/BF00331864. [DOI] [PubMed] [Google Scholar]
  5. Brown T. C., Harris P. V., Boyd J. B. Effects of radiation on the survival of excision-defective cells from Drosophila melanogaster. Somatic Cell Genet. 1981 Nov;7(6):631–644. doi: 10.1007/BF01538753. [DOI] [PubMed] [Google Scholar]
  6. Chan L. N., Gehring W. Determination of blastoderm cells in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2217–2221. doi: 10.1073/pnas.68.9.2217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferro W. Studies on mutagen-sensitive strains of Drosophila melanogaster. V. Biochemical characterization of a strain (ebony) that is UV- and X-ray sensitive and deficient in photorepair. Mutat Res. 1985 May;149(3):399–408. doi: 10.1016/0027-5107(85)90156-3. [DOI] [PubMed] [Google Scholar]
  8. Harris P. V., Boyd J. B. Pyrimidine dimers in Drosophila chromatin become increasingly accessible after irradiation. Mutat Res. 1987 Jan;183(1):53–60. doi: 10.1016/0167-8817(87)90045-9. [DOI] [PubMed] [Google Scholar]
  9. Maurer R., Osmond B. C., Botstein D. Genetic analysis of DNA replication in bacteria: dnaB mutations that suppress dnaC mutations and dnaQ mutations that suppress dnaE mutations in Salmonella typhimurium. Genetics. 1984 Sep;108(1):25–38. doi: 10.1093/genetics/108.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Resnick M. A. A photoreactivationless mutant of Saccharomyces cerevisiae. Photochem Photobiol. 1969 Apr;9(4):307–312. doi: 10.1111/j.1751-1097.1969.tb07294.x. [DOI] [PubMed] [Google Scholar]
  11. Ryo H., Kondo S. Photoreactivation rescue and hypermutability of ultraviolet-irradiated excisionless Drosophila melanogaster larvae. Proc Natl Acad Sci U S A. 1986 May;83(10):3366–3370. doi: 10.1073/pnas.83.10.3366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sancar A., Franklin K. A., Sancar G. B. Escherichia coli DNA photolyase stimulates uvrABC excision nuclease in vitro. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7397–7401. doi: 10.1073/pnas.81.23.7397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Setlow R. B., Setlow J. K. Effects of radiation on polynucleotides. Annu Rev Biophys Bioeng. 1972;1:293–346. doi: 10.1146/annurev.bb.01.060172.001453. [DOI] [PubMed] [Google Scholar]
  14. Sutherland B. M., Rice M., Wagner E. K. Xeroderma pigmentosum cells contain low levels of photoreactivating enzyme. Proc Natl Acad Sci U S A. 1975 Jan;72(1):103–107. doi: 10.1073/pnas.72.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yamamoto K., Satake M., Shinagawa H. A multicopy phr-plasmid increases the ultraviolet resistance of a recA strain of Escherichia coli. Mutat Res. 1984 Jan;131(1):11–18. doi: 10.1016/0167-8817(84)90042-7. [DOI] [PubMed] [Google Scholar]
  16. Yegorova L. A., Levin V. L., Kozlova M. A. Heritability of UV-sensitivity and photo-reactivation ability in Drosophila embryos. Mutat Res. 1978 Feb;49(2):213–218. doi: 10.1016/0027-5107(78)90159-8. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES