Figure 7.

Characterization of chl breakdown in pao1. A, Detached pao1 and Col-0 leaves after 5-d senescence in the dark. B, Degradation of chl in Col-0 (black) and pao1 (gray). C, Accumulation of pheide a in response to dark incubation of Col-0 (black) and pao1 (gray) leaves. D and E, Determination of ion leakage as a measure for cell death in pao1 (D) and Col-0 (E) leaves. Before re-exposure to the light for up to 8 h, leaves were incubated in the dark for 0 (black circles), 3 (gray circles), or 5 (white circles) d. Results of a single representative experiment are shown. Data are means of three replicates. Error bars indicate sd.