Figure 2.
PEX12 gene structure and knockout phenotypes. A, Schematic representation of PEX12, showing exons as white boxes and introns as single lines. The triangle indicates the position of the T-DNA insertion. Primers shown were used for genotyping. B and C, Immature siliques of wild-type and heterozygous plants. Arrows point to mutant seeds. Bars = 0.5 mm. D, PCR genotyping of embryo DNA. Lanes 1 to 2, Normal embryos; lanes 3 to 4, abnormal embryos. Primers used for 1 and 3, P12F/P12R; for 2 and 4, LB/P12R.