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Southern-blot hybridization showing remethylation of cytosines in the SR-VI subregion in regenerated plants and their progeny. Results for two out of 15 regenerated plants (R01 and R02) and two out of 10 progeny plants (S01 and S02) are presented. Genomic DNAs digested with MboI (−) and MboI plus HpaII (+) enzymes were probed with the IGS probe. Arrows indicate positions of hypomethylated fragments. The degree of hypomethylation was quantified as described in Figure 3.
