Table I.
Enzymes and probes used for the analysis of cytosine methylation in 35S rDNA
| Region Analyzed | Enzyme Limiting the Regiona | Methylation-Sensitive Enzyme | DNA Probe | No. of Sites/Region Analyzed |
|---|---|---|---|---|
| IGS-SR VI | EcoRV | ClaI (ATmCGAT)b | 18S | 4–7c |
| IGS-SR VI | MboI | HpaII (mC mCGG) | A1/A2 | 1 |
| IGS-SR VII | EcoRV | PstI (mCTGmCAG) | 18S | 1c |
| IGS-SR II | BstNI | TaiI (AmCGT) | 26S | 17 |
| 18S | EcoRV | SalI (GTmCGAC) | 18S | 1 |
| 26S | - | XhoI (CTmCGAG) | 26S | 1 |
| 26S | BstNI | NciI (CmCSGG) | 26S | 6 |
a
Enzymes are not sensitive to the presence of 5-methylC (see Fig. 1).
b
Recognition sequences are in parentheses (m, sensitive cytosine).
c
Sites are present in the N. tomentosiformis-derived gene families, but not in those of N. sylvestris origin.