Figure 3.
NpPDR1 silencing by RNA interference. A, Microsomal fractions prepared from the leaf epidermis (LE) or from a leaf section 16 h after sclareol infiltration (LScl) of wild-type or NpPDR1-silenced plants were subjected to western blotting using the indicated antibodies. wt, Wild-type; 3, 6, 8, 9, PDR1-silenced lines. B, RNA was prepared from a leaf section 96 h after P. syringae pv tabaci infiltration and subjected to northern-blot analysis using primers specific for the indicated genes. C, Microsomal fractions prepared from the samples analyzed in B were subjected to western blotting using the indicated antibodies.