Figure 7.

Induction of processing proteases by MeJA in N. attenuata. Plants were elicited with MeJA, and the local (Loc) and systemic (Sys) leaves were harvested from three replicate plants on each of eight consecutive days after elicitation. The content (mean ± se) of VPE and subtilisin-like proteases was quantified in an activity assay with the specific substrates Z-AAN-AMC and N-LP-FRET, respectively. The relative activity levels of both VPE and subtilisin-like proteases are expressed relative to those of unelicited control leaves harvested on the same day.