Table I.

Quantification of TPIs isolated from leaf tissue of N. attenuata

The recovery of RP-HPLC purified TPIs was determined by protein assays, and the relative distribution of isoinhibitors was calculated from the yield of N-terminal sequencing signals. The TPIs were compared for MeJA-elicited and control plants of the Utah genotype, which produces TPIs (wild type), and the Arizona genotype, which had been transformed with the TPI precursor gene of the wild-type genotype under control of a constitutive promotor (S++).

			Distribution of Isoinhibitors
Genotype	Elicitation	Total TPI Yield	Irregular Processinga	Trimming Ratiob
			%	EEK-/EK-	DRI-/RI-
		μgg−1 dry wt
Wild type	Control	56 ± 1	0	0/0	93/7
	MeJA	370 ± 10	24	81/19	68/32
S++	Control	87 ± 4	0	0/0	n.d.c
	MeJA	79 ± 3	0	0/0	n.d.c

^aThe relative content of irregularly processed TPIs in total the TPI pool. Irregular processing was defined by unremoved LP at the N terminus (EEK- or EK- termini).

^bThe relative distribution of trimmed isoinhibitors. The trimming indicates one residue variability at the N terminus of irregularly (EEK- and EK- termini) and regularly processed (DRI- and RI- termini) TPIs.

^cn.d., Not determined.