Table II.

Characterization of TPIs from the wild-type genotype

The assignment of the amino acid sequence of TPI peptides was based on N-terminal sequencing. The molecular mass was determined by MALDI-TOF mass spectrometry, and the predicted mass was calculated from cDNA sequence. The core sequence corresponds to [RIC…CPR] residues of the individual TPI domains (Fig. 3). The analyzed TPI fractions (see Fig. 2 for numbering) were isolated from unelicited plants (control) or plants elicited with MeJA.

Fraction	Mass Observed	Mass Calculated	N-Terminal Sequence	TPI Domain	Assigned Structure
	D	D
Control 1	5758	5758	DRIXT	B	D[core]S
		5759	DRIXT	D	D[core]
Control 2	5670	5671	DRIXT	B	D[core]
	5727	5729	DRIXT	F	D[core]
	5748	5744	DRIXT	C or E	D[core]
Control 3	5833	5831	DRIXT	C	D[core]S
		5831	DRIXT	F	D[core]T
Control 4	5743	5743	DRIXT	C or E	D[core]
	5845	5845	DRIXT	E	D[core]T
		5847		D	D[core]S
Control 5	5646	5644	RIXTN	D	[core]
		5643	RIXTN	B	[core]S
	5760	5760	DRIXT	D	D[core]
		5759	DRIXT	B	D[core]S
Control 6	6142		AXPRN	A	n.a.a
	6265		KAXPR	A	n.a.
Control 7	5130–6265		AXPRN/KAXPR	A	n.a.
MeJA 1	6231	6226	EKKND	F	EEKKND[core]
	6332	6330	EKKND	C	EKKND[core]S
		6330		F	EKKND[core]T
	6391	6388	EEKKN	D	EEKKND[core]
		6387		B	EEKKND[core]S
	6460	6459	EEKKN	C	EEKKND[core]S
		6459		F	EEKKND[core]T
MeJA 2	5557	5556	RIXTN	B	[core]
	5617	5614	RIXTN	F	[core]
	5672	5671	DRIXT	B	D[core]
	5730	5729	RIXTN	E	[core]T
		5731	RIXTN	D	[core]S
		5729	DRIXT	F	D[core]
	5761	5760	DRIXT	D	D[core]
		5759	DRIXT	B	D[core]S
	5831	5830	DRIXT	C	D[core]S
		5830		F	D[core]T
	6372	6372	EEKKN	C or E	EEKKND[core]
	6475	6475	EEKKN	D	EEKKND[core]S
		6477	EEKKN	E	EEKKND[core]T
MeJA 3	5834	5831	DRIXT	C	D[core]S
		5831		F	D[core]T
MeJA 4	5630	5628	RIXTN	C or E	[core]
	5742	5743	DRIXT	C or E	D[core]
MeJA 5	5629	5628	RIXTN	C or E	[core]
	5742	5743	DRIXT	C or E	D[core]
	5846	5845	DRIXT	E	D[core]T
		5847		D	D[core]S
	5733	5729	DRIXT	F	D[core]
		5730	RIXTN	E	[core]T
		5732		D	[core]S
MeJA 6	5646	5644	RIXTN	D	[core]
		5643		B	[core]S
	5761	5760	DRIXT	D	D[core]
		5759		B	D[core]S
MeJA 7	6137		AXPRN	A	n.a.
	6265		KXPR	A	n.a.
MeJA 8	5130–6265		KAXPR/AXPRN	A	n.a.

^an.a., Not assigned.