. 2005 Sep;139(1):375–388. doi: 10.1104/pp.105.064006

Table III.

Characterization of posttranslational modifications of the two-chain TPI

The N termini of the separated chains were determined by sequencing. The C termini were identified by assigning MALDI-TOF mass spectrometry data to cDNA sequence. The A2 chain was characterized after removal of N-terminal pyroglutamic acid (<Q) and N-linked oligosaccharide of paucimannosidic type.

Peak	Mass Observed	Mass Calculated^a	N-Terminal Sequence^b	Assigned Structure
	D	D
1-K	2240	2239	KACPRNCDGRIAYEICPR	KACPRNCDGRIAYEICPR
1-A	2114	2111	ACPRN	ACPRNCDGRIAYEICPR
A2	4502		(<Q)ICTN^c	n.a.^d
	3490^e	3487		<QICTNCCAGTKGCNYFSANGTFICEGESEY

Mass calculated with carboxymethylated Cys residues.

Cys residues identified as carboxymethyl Cys.

Sequence determined after treatment with pyroglutamate aminopeptidase.

n.a., Not assigned.

Mass determined after treatment with N-glycosidase A.