Table IV.

Characterization of TPIs from S++ plants in which the TPI production of the Arizona genotype was restored by transformation

The assignment of the amino acid sequence of the TPI peptides is based on N-terminal sequencing. The molecular mass was determined by MALDI-TOF mass spectrometry, and the predicted mass was calculated from cDNA sequence. The core sequence corresponds to [RIC…CPR] residues of the individual TPI domains (Fig. 3). The analyzed TPI fractions (see Fig. 10 for numbering) were isolated from unelicited plants (control) or plants elicited with MeJA.

Fraction	Mass Observed	Mass Calculated	N-Terminal Sequence	TPI Domain	Assigned Structure
	D	D
Control 1	5673	5671	DRIXT	B	D[core]
	5732	5729	DRIXT	F	D[core]
MeJA 1	5673	5671	DRIXT	B	D[core]
	5731	5729	DRIXT	F	D[core]
Control 2	5832	5831	DRIXT	C	D[core]S
		5831	DRIXT	F	D[core]T
MeJA 2	5832	5831	DRIXT	C	D[core]S
		5831	DRIXT	F	D[core]T
Control 3	5633	5628	RIXT	C or E	[core]
	5748	5743	DRIXT	C or E	D[core]
	5841	5844	DRIXT	E	D[core]T
		5846		D	D[core]S
MeJA 3	5631	5628	RIXT	C or E	[core]
	5746	5743	DRIXT	C or E	D[core]
	5848	5844	DRIXT	E	D[core]T
		5846		D	D[core]S
Control 4	5644	5644	RIXTN	D	[core]
		5643	RIXTN	B	[core]S
	5759	5760	DRIXT	D	D[core]
		5759	DRIXT	B	D[core]S
MeJA 4	5646	5644	RIXTN	D	[core]
		5643	RIXTN	B	[core]S
	5762	5760	DRIXT	D	D[core]
		5759	DRIXT	B	D[core]S
Control 5	6140		AXPRN	A	n.a.a
	6270		KAXPR	A	n.a.
MeJA 5	6138		AXPRN	A	n.a.
	6269		KAXPR	A	n.a.

^an.a., Not assigned.