Figure 2.

Analysis of nucleotides flanking consensus CATG motifs and CCGAC motifs in VAA- and cold-regulated promoters. A, All 8-mer oligos enriched the VAA promoter set that included CATG were evaluated for the two flanking nucleotides in both orientations. The dotted line shows expected frequency by random distribution. The black bars represent the sequences with a significantly skewed frequency (P < 0.05), whereas the shaded bars represent those with P > 0.05. B, All the CCGAC sequences within significant 8-mers from cold long-term up-regulated genes were examined for the flanking nucleotides. If more than two distinct CCGAC motifs were present in the promoters, then CCGACNT was taken for the evaluation. Activation level was calculated by peak average difference × induction at 24 h, according to the values reported by Fowler and Thomashow (2002).