Diurnal changes of isoprene biosynthesis-related parameters in leaves of 4-month-old poplar trees during two consecutive days in August 2001. A, Transcript levels of PcDXR, PcISPS, and leaf temperature (bold gray line); B, DMADP levels and isoprene emission rate; C, PcISPS activity and PPFD (bold gray line; n = 4 ± se; gas exchange measurements n = 2 ± range). Shaded regions symbolize dark periods.