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. 2025 Apr 14;12:1530765. doi: 10.3389/fvets.2025.1530765

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Copyright © 2025 Chen, Fan, Huang, Zhou, Chen, Chen, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Isolation, identification of the virus and biological characteristics of the virus strain. (A) Indirect immunofluorescence analysis of PK-15 cells infected by GDWS2 strain. The green signal is gE antibody. (B) Morphological characteristics of GDWS2 strain under an electron microscope (bar = 100 nm). (C) Cytopathic effect of GDWS2 strain on cells from different sources. (D) One-step growth curves of different strains. (E) Sizes of plaques caused by different PRV strains on PK-15 cells quantified using the ImageJ software (n = 6). (F) Plaque morphologies of different PRV strains on PK-15 cells. The significance level was set at p < 0.05 (∗), p < 0.01 (∗∗), and p < 0.001 (∗∗∗).