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. 2025 Apr 14;12:1530765. doi: 10.3389/fvets.2025.1530765

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Copyright © 2025 Chen, Fan, Huang, Zhou, Chen, Chen, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of viral virulence by LD50 in rabbit models. (A) The survival curve of experimental ribbit inoculated with different PRV strains or DMEM. (B) Detection of viral DNA in rabbit tissues infected with different doses of the JM strain by fluorescence quantification. (C) Detection of viral DNA in rabbit tissues infected with different doses of the GDWS2 strain by fluorescence quantification. (D) Comparison of viral DNA in various organs of different strains at an infection dose of 1 × 10⁵ TCID50. (E) Comparison of viral DNA in various organs of different strains at an infection dose of 1 × 10⁴ TCID50. (F) Comparison of viral DNA in various organs of different strains at an infection dose of 1 × 10³ TCID50. The significance level was set at p < 0.05 (∗), p < 0.01 (∗∗), and p < 0.001 (∗∗∗).