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. 2025 Apr 14;12:1530765. doi: 10.3389/fvets.2025.1530765

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Copyright © 2025 Chen, Fan, Huang, Zhou, Chen, Chen, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of viral virulence using pig models. (A) Survival curve of experimental pigs inoculated with different PRV strains or DMEM. (B) Rectal temperature of pigs inoculated with different PRV strains or DMEM at different days post inoculation. (C) Detection of viral DNA in the nose and mouth swabs collected from pigs inoculated with different PRV strains at different days post inoculation by real-time quantitative PCR. (D) Detection of viral DNA in anal swabs collected from pigs inoculated with different PRV strains at different days after inoculation by real-time quantitative PCR. (E) Detection of viral DNA in blood collected from pigs inoculated with different PRV strains at different days after inoculation by real-time quantitative PCR. (F) Detection of viral DNA in different pig tissues by real-time quantitative PCR. The significance level was set at p < 0.05 (∗), p < 0.01 (∗∗), and p < 0.001 (∗∗∗).