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. 2025 Mar 29;16(7):2233–2249. doi: 10.7150/jca.105120

Figure 1.

Figure 1

RBM39 is overexpressed in CRC tissues and correlates with a poor prognosis. (A) The online tool (http://www.sxdyc.com/) was used to analyze RBM39 mRNA expression across various tumors and normal tissues using TCGA datasets. (B) The GEPIA online tool was employed to analyze RBM39 mRNA expression in normal and CRC tissues using data from TCGA datasets. (C) The mRNA expression levels of RBM39 were measured by qRT-PCR in 10 pairs of colorectal cancer tissues and corresponding adjacent normal tissues (n = 3). (D) The protein expression levels of RRBM39 were measured by Western blot in 10 pairs of colorectal cancer tissues and corresponding adjacent normal tissues. (E) The mRNA levels of RBM39 were assessed in CRC cell lines (Caco2, RKO, SW620, SW1116, HT29, SW480, HCT116) and the normal colorectal cell line NCM460 using qRT-PCR (n = 3). (F) RBM39 protein levels were assessed in CRC cell lines (Caco2, RKO, SW620, SW1116, HT29, SW480, HCT116) and the normal colorectal cell line NCM460 using western blot. (G) Representative RBM39 is highly expressed in CRC tissues and compared with paired adjacent normal tissues using immunohistochemistry. Images under 400× magnification, scale bars, 50 µm. (H) The Kaplan-Meier online tool was utilized to perform survival analysis of CRC patients based on RBM39 mRNA expression. (N = adjacent normal tissue, T = tumor tissue, data are presented as the mean ± SD, the differences between two groups were analyzed using Student's t-test, *p < 0.05 **p <0.01, ***p <0.001).