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ELISA results of Atf4, Ep300, Sphk1 and Mmp9 in serum and lung tissue between Sham, LIR, LIR + DEX, LIR + ATIP and LIR + DEX + ATIP groups. (n = 6 per group) (A) Atf4, (B) Ep300, (C) Sphk1 and (D) Mmp9 levels in serum. (E) Atf4, (F) Ep300, (G) Sphk1 and (H) Mmp9 levels in lung tissue.
Notes: *P < 0.05; **P < 0.01; ***P < 0.001.
Abbreviations: ELISA, Enzyme-Linked Immunosorbent Assay; LIR, limb ischemia-reperfusion; DEX, dexmedetomidine; ATIP, atipamezole.
