Fig. 5. Antimicrobial effect of NM102 on Gram-negative bacteria during NO stress.

Strains were grown to exponential phase in LB medium. The bacteria solution was prepared in RPMI medium and dispatched in 96-wells plate. Bacteria were exposed for 4 h at 37 °C to 50 µL of increasing concentrations of NM102 alone A K. pneumoniae DOU or with NOC-5 as a NO donor, B K. pneumoniae DOU, C E. coli ATCC 25922, D P. aeruginosa CIP27853). The bacteria survival rate was calculated by normalizing bacteria load against control without NM102. The results reported are mean ± SD of four independent experiments each in triplicates, P values are calculated against the condition without NM102, using One Way ANOVA (****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, the exact P values are provided as source data). IC₅₀ was computed using Graph Pad 7.05. Source data are provided as a source data file.