Fig. 8. NM102 does not impact the gut microbiome.

Gut microbiome diversity is unaffected by NM102. Mice were infected with K. pneumoniae and left untreated or treated with NM102 nanoformulation (NF-NM102) or the empty nanoformulation (NF) as control. After 24 h, feces were collected and the microbiome diversity was analyzed by 16S rRNA gene sequencing. Relative abundance of bacterial families (A), Shannon index (B), and weighted and unweighted UniFrac distances (C) were determined. The bar colors represent bacterial families with mean abundance >1%. Each dot (B, C) and each column (A) represents the value obtained for one mouse. Error bars in Fig. 6B represent mean ± SEM. The sample size in Fig. 6 A, B, C is n = 7 mice per group. The statistical test in Fig. 6B is permutational anova (PERMANOVA). The statistical test in Fig. 6C is a two-tailed Wilcoxon test with Benjamini-Hochberg correction for multiple comparisons. Source data are provided as a source data file.