Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | AAV-CAG-PFE3-P2A-GFP (plasmid) | This paper | RRID:Addgene_236178 | AAV version of constitutive PFE3 |
| Recombinant DNA reagent | AAV-TRE-PFE3-P2A-GFP (plasmid) | This paper | RRID:Addgene_236180 | AAV version of Tet-inducible PFE3 |
| Recombinant DNA reagent | AAV-CAG-RandE3-P2A-GFP | This paper | RRID:Addgene_236181 | AAV version of constitutive control plasmid |
| Recombinant DNA reagent | AAV-TRE-RandE3-P2A-GFP | This paper | RRID:Addgene_236179 | AAV version of inducible control plasmid |
| Recombinant DNA reagent | tagRFP-GPHN.FingR-PhoCl (plasmid) | This paper | RRID:Addgene_236183 | Half of photoactivatable GFE3 (paGFE3) |
| Recombinant DNA reagent | PBP-E3 (plasmid) | This paper | RRID:Addgene_236182 | Other half of paGFE3 |
| Recombinant DNA reagent | TRE-PFE3-HA | This paper | RRID:Addgene_236114 | Tet-inducible-PFE3-HA |
| Recombinant DNA reagent | tagRFP-GPHN.FingR-HaloTag (plasmid) | This paper | RRID:Addgene_236112 | Half of chemically inducible GFE3 (chGFE3) |
| Recombinant DNA reagent | eDHFR-E3 | This paper | RRID:Addgene_236113 | Other half of chGFE3 |
| Antibody | Anti-PSD-95 (mouse monoclonal) |
Commercially available. | Novus Biologicals cat. # NB300-556 | 1:3000 |
| Antibody | Anti-GluA1 (rabbit polyclonal) |
Commercially available. | Millipore Sigma cat. # ABN241 | 1:1000 |
| Antibody | Anti-GFP (chicken polyclonal) |
Commercially available. | Fisher Scientific cat. # NC9510598 | 1:10,000 |
| Antibody | Anti-HA (rabbit polyclonal) | Commercially available. | Cell Signaling cat. # 3724 | 1:1000 |
| Antibody | Anti-MYC (chicken polyclonal) | Commercially available. | Novus Biologicals cat. #NB600-334 | 1:1000 |
| Genetic reagent (M. musculus) | CCK-Cre | Commercially available. | Jackson Laboratories Ccktm1.1(cre)Zjh/J | Strain 012706 |
| Genetic reagent (M. musculus) | Ai27(RCL-hChR2(H134R)/tdT)-D | Commercially available. | Jackson Laboratories B6.Cg-Gt(ROSA)26Sortm27.1(CAG-COP4*H134R/tdTomato)Hze/J | Strain 012567 |